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The basis for quality and reproducibility of the analysis

The key to reproducible results by development under standardized conditions

Three kits to cover a wide range of cAMP concentrations.After the introduction of IP-One, the first HTS immunoassay that can easily detect inositol(1)phosphate (IP1), a stable second messenger which tightly correlates with Gq-coupled receptor activity, the optimization of cAMP assays with the new HTRF® acceptor d2 offers a complete GPCR platform.The upgraded cAMP dynamic 2 and cAMP femto 2 assays, along with the new cAMP HiRa…

An easy way to assess tag accessibilityThe production of fusion proteins often raises the issues not only of their structural integrity after isolation, but also of the accessibility of the associated tag. In the case of GST or 6HIS tagged proteins, this verification can be achieved with HTRF® GST and 6HIS check kits. These two assays are competitive immunoassays in which the anti-GST or anti-6HIS Cryptate conjugates bind dire…

A rapid and sensitive homogeneous assay for cortisol assessmentThe HTRF® cortisol assay can be used for the quantification of cortisol in rodent serum, or directly on cells. This assay has also been validated for 11-beta HSD1 screening on whole cells or microsomes.The assay requires only 2 reagent additions, followed by an incubation period of 2 hours at room temperature. It has a detection limit of 70 pg/mL (e.g. 2 fmol/well…

A straightforward tool for biomolecular labeling with Europium cryptateThe Cryptate labeling kit includes all the necessary materials to allow rapid Cryptate labeling of various biomolecules and purification. The labeling reagent Eu3+-TBP-NHS Cryptate is suitable for labeling peptides, proteins, and oligonucleotides, as well as small molecules containing amino groups (N-terminus or lysine residues). The labeling procedure requ…

A range of HTRF® conjugates for assay developmentHTRF®  toolbox assembles a panel of detecting reagents such as Streptavidin-XLlent!, anti-tag antibodies like Eu-Cryptate anti-GST, 6HIS, DNP and anti species conjugated antibodies offering many possibilities for the study of molecular mechanisms. All reagents are available as Cryptate-Europium or XL665 (the two HTRF tracers) conjugates, to allow easy assay development. These re…

A simple and straightfor­ward procedure for measuring functional kinase activity.The Eu(K) labeled antibody and XL665 conjugate are added simul­taneously after the enzymatic step. Results are available after a 2-hour incubation at room temperature. The two reagents are stable in solution, even in an excess of EDTA (0.5M), while the assay remains stable for >24 hours. An extended list of Eu3+-Cryptate labeled phos­pho-specific…

An homogeneous fluorescence assay for PGE2Prostaglandin E2 (PGE2) is a primary product of arachidonic metabolism in many cells. Under inflammatory stimuli, PGE2 is de novo synthesized by three enzymatic steps via the cyclooxygenase (COX) pathways. PGE2 production is commonly used in the detection of COX-1 and COX-2 activity. In the PGE2 HTRF® assay, free PGE2 from the sample competes with XL665 labeled PGE2 for binding to a Cr…

The assay involves two monoclonal antibodies, one labeled with Cryptate and the other with XL665, that recognize distinct determinants. The assay is performed in a single step and requires only a short incubation period. Detection limit of the assay is 0.20 ng/mL and the linear range covers 0.3 to 10 ng/mL. The assay has been validated on various biological material including isolated perfused pancreas, plasma, and pancreatic…

Streamlined single plate protocols including all stimulation and cytokine quantification.The HTRFâ cytokine kit line includes TNFa, IFNg, IL1b, IL6 and IL8 assays. These assays feature no separation steps, a high sensitivity and dynamics, and no use of isotopes. Procedures are performed on a single plate with simultaneous cell stimulation and cytokine detection. They can all be run under a similar experimental procedure stream…

A homogeneous single plate assay for the sensitive quantification of histamine.Histamine is a small biogenic amine that plays an important role in several physiological and pathophysiological processes. The histamine HTRF® assay is based on the competition between sample histamine and XL665-labeled histamine for binding to a Cryptate-labeled antibody. The amount of histamine detected is inversely proportional to the specific f…

A streamlined assay for human monoclonal antibody screening.The human Fc check kit is a competitive immunoassay involving a Cryptate conjugated polyclonal antibody specific to human Fc IgGs and XL665 coupled human IgGs. This homogeneous assay enables a precise assessment of all human IgG isotypes within 3 hours at room temperature and is particularly adapted to human monoclonal antibody (MAb) screening. Like all other HTRF® as…

A new secondary messenger for Gq coupled receptor investigation.Cisbio’s IP-One assay, based on its proprietary HTRF® technology, is the first HTS immunoassay that can easily detect inositol(1)phosphate (IP1), a stable second messenger which tightly correlates with Gq-coupled receptor activity. This assay displays all the characteristic advantages of an HTRF® system: totally homogeneous, detection via fluorescence, signal stab…

A unique set of reagents for the investigation of ubiquitin.The HTRF® Ubiquitin toolbox assembles all the reagents for flexible assay development and HTS of each step of the ubiquitin system. Ubiquitin is directly labeled with the Eu3+-Cryptate. This conjugate is directly incorporated into the ubiquitin chain during the enzymatic step. Upon the addition of the XL665 conjugate, the HTRF® signal is proportional to the level of u…

A team of experts for outsourcing your assay developmentsOur Service Team at Cisbio international is highly trained in protein chemistry and fluorescent technology, and offers you its expertise in assay development to build HTRF® assays adapted to your specific targets or to convert existing assays to HTRF® technology.Assays may be developed using HTRF® toolbox reagents, custom labeled molecules or a combination of both. Most…

A fast and reliable process for biomolecule labelingIn many circumstances, assay development and HTS involve specific labeling of the various assay components. Cisbio international’s labeling service is an excellent alternative for the labeling of proteins, peptides, oligonucleotides, or other biomolecules either with Cryptate, XL665, biotin or DNP when you do not have the time or the facilities to label them yourself.Two type…

jetPEI™ , the powerful transfection reagent for plasmids and   oligonucleotides delivery Extremely efficient Highly reproducible results Easy to use: with/without serum, no medium change & no washes Fast HTS protocol

High productivityVersatile: efficient in both HEK 293 & CHO synthetic mediaComplies with new regulatory requirements for large scale production

Dimension P1 The Dimension-P1™ Raman System is the first of LSI's Dimension series products. The CE and UL Certified Dimension-P1™ is an ultra-high performance Raman spectrometer achieved through the integration of proprietary optics and unique software solutions. The high sensitivity of its proprietary fiber-bundle probe system and  high light throughput spectrometer, combined with the unsurpassed performance of…

Dimension P2 The Dimension-P2™ Raman System represents a true portability solution for high performance Raman spectroscopy. The Dimension-P2™ comes with LSI's Vector Raman Probe™ and the powerful RamanSoft for data acquisition, data processing, and data analysis. The system includes the External Sampling Module allowing the user to determine a wide range of potential applications and operational conditions. The Dime…

The Dimension-M1™ Raman MicroscopeLambda Solutions now provides a unique coupling of its Dimension series Raman systems to Nikon or Olympus microscopes. The result is a high performance Raman microscope at an extraordinary value. The Dimension-M1™ is available as an integrated unit, or as a Dimension-P1™ Raman System with the addition of a microscope and a Raman Microscope Adapter.  This system is available with the high spati…

The Vector Raman Probe™ The Vector Raman Probe™ is a high performance optical device designed to yield high sensitivity for all applications in Raman spectroscopy with flexibility of use. The probe comes with >OD6 laser rejection and a cutoff wavenumber <150 cm-¹ from the 785 nm laser line. The Vector Raman Probe™ comes with SMA connectors for flexible detachable fiber use. The stainless steel cover allows for easy hand-held…

The Vector NIR ProbesThe Vector NIR probes are designed for diffuse reflectance spectroscopy applications. Ideal for research and QA/QC in chemical, agricultural, food and pharmaceutical industries. Interfaces with most existing FTIR, AOTF, and Dispersive Spectrometers for applications requiring high sensitivity.Proprietary optic design: low internal light reflection with exceptional light collection efficiency ensuring high s…