The universal reverse primer is designed for use with the Human U6 forward primer provided in our High-Specificity miRNA QRT-PCR Detection Kit for a complete, validated assay requiring no upfront design work. The Kit delivers sensitive and reproducible detection of miRNA with high specificity.
Our miRNA qRT-PCR detection system enables accurate and real-time PCR quantification for miRNA. The kit is a high-specificity reagent system that delivers sensitive and reproducible results. The kit elongates miRNAs in a polyadenylation reaction and then reverse transcribes the polyadenylated RNA into QPCR-ready cDNA.
Agilent miRNA qPCR Detection Reagents are high-specificity reagents for the polyadenylation of microRNAs (miRNAs) from total RNA. The miRNA qPCR Detection Reagents also enable the synthesis of first-strand cDNA from the tailed miRNAs.
The Agilent SideStep II Lysis and Stabilization Buffer uses a single-tube, solution-based format, rather than a column-based format. The patent-pending solution lyses cells in minutes and releases both RNA and DNA molecules into the solution. This immediately stabilizes nucleic acids without further processing required.
The SideStep mRNA Enrichment Kit offers fast and efficient delivery of mRNA directly from cells without purifying total RNA. Save time and money with intuitive 30-minute methods for research applications.Â
Our SideStep II QRT-PCR products deliver efficient cell lysis and sensitive quantitative gene expression analysis without RNA purification. Utilizing Agilent’s Brilliant QRT-PCR technology, multiple samples can be screened quickly and easily. The one-step process results in RNA stabilized for up to 6 months.
Our SideStep Lysis and Stabilization Buffer ensures accurate gene expression analysis by eliminating sample loss and degradation. The proprietary buffer lyses cells and immediately stabilizes the released nucleic acid for months. Several formats are available to enhance research versatility.
Data acquisition and analysis performed by the 2100 Bioanalyzer Expert Software package is unbiased and highly reproducible. The sample fragment size and sample concentration are automatically provided, with further customized analysis tools available for specific assays.
The integrated, SPD bench-top SpeedVac concentrator is ideal for aqueous and non-aggressive solvent evaporation as well as DNA applications.It has an oil-free vacuum pump and refrigerated vapor trap incorporated making it a fully integrated system.Product Detail The SPD integrated SpeedVac has a vacuum pump, cold-trap and concentrator fully integrated into one convenient system. Digital programming enhances the simplicity of…
StrataPrep Plasmid Miniprep Kits, a rapid, phenol-free method for purifying plasmid DNA from bacterial cultures. Bacteria is first pelleted, lysed, and neutralized. Precleared lysate is then mixed with a DNA binding solution and subjected to microspin. Once bound, the purified DNA is washed, eluted, and ready for use.
StrataPrep Plasmid Miniprep Kits, a rapid, phenol-free method for purifying plasmid DNA from bacterial cultures. Bacteria is first pelleted, lysed, and neutralized. Precleared lysate is then mixed with a DNA binding solution and subjected to microspin. Once bound, the purified DNA is washed, eluted, and ready for use.
Agilent’s RNA spike-In kit lets researchers efficiently monitor their microarray workflow for linearity, sensitivity, and accuracy. Each spike mix in these one-color and two-color RNA spike-in kits contains 10 in vitro synthesized, polyadenylated transcripts in predetermined ratios.
Agilent’s RNA spike-In kit lets researchers efficiently monitor their microarray workflow for linearity, sensitivity, and accuracy. Each spike mix in these one-color and two-color RNA spike-in kits contains 10 in vitro synthesized, polyadenylated transcripts in predetermined ratios.
Agilent's Ampicillin tabs provide a high quality tablet option to select for ampicillin-resistant bacteria during analysis. The Ampicillin ready made solution is for research use only and provided in powder form. The tablets correct for 6-aminopenicillanic acid, 6-APA, and are used to reduce satellite colonies when selecting for ampicillin-resistant bacteria. Turbo Amp Antibiotic is used to ensure precise results.
Isopropyl-β-D-thiogalactoside (IPTG) is used in combination with X-gal to detect β-galactosidase activity in blue/white selection assays. When the appropriate vectors are used, blue white screening is an important tool for screening and selecting relevant colonies.
Ampicillin, IPTG, and X-Gal in convenient packaging.  G418 Sulfate  Hybromycin B
Agilent X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, BCIG) is a premium quality chromogenic substrate of β-Galactosidase. It is used with cloning systems that utilize β-galactosidase activity as an indicator of successful cloning.
The Agilent miRNA Spike-in kit allows researchers to monitor the miRNA labeling and hybridization reactions. The Spike-in kit contains a labeling and hybridization spike-in, which correspond to complementary probe sequences on the microarrays. The Spike-ins and probes are designed using Drosophila.
The Paq5000 DNA polymerase is a robust Taq substitute that is derived from a Pyrococcus species. This enzyme provides equivalent and often better performance to endpoint Taq DNA polymerase amplification and can be substituted easily into many Taq-based procedures.  Robust, cost-effective alternative to Taq  Equal to better yield with faster cycling conditions  Amplify up to 6 kb genomic DNA targets  Easily substituted…
The MicroModulyo is a compact, space saving personal freeze dryer. Perfect for academic research where bench space is premium. A wide range of accessories give you complete flexibility. Product Detail The MicroModulyo Freeze Dryer is a space saving, cost effective, 1.5 liter ice condenser designed specifically for the small volume user. It features an all stainless steel, "e;coil-in-chamber"e; ice condenser with an easy-…
PCR Polishing Kit enables an increase in blunt-ended cloning efficiencies associated with polymerase chain reaction (PCR)-generated fragments. It polishes the ends of the 3’ overhang extensions of polymerase-generated DNA fragments directly from PCR amplification and can completely fill in 5’ overhangs to generate blunt ends.
The TaqPlus Precision polymerase mixture is an optimized blend of Stratagene cloned Pfu DNA polymerase and Taq2000 DNA polymerase, a highly purified, recombinant version of Taq DNA polymerase. The TaqPlus Precision PCR system exhibits the highest replication accuracy achieved using DNA polymerase mixtures.
The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping. The pBluescript II phagemids have an extensive polylinker with 21 unique restriction enzyme recognition sites.  New cDNA synt…
The StrataCooler LP Benchtop Cooler has a capacity of 32 1.5 mL tubes and weighs 1.2 kg. The StrataCooler LP Benchtop Cooler provides the most protection for your enzymes in the freezer or on the benchtop. It maintains -15 for at least 2 hours.
The LumiMax superoxide anion detection kit qualitatively detects the presence of superoxide anions and superoxide dismutase in cell cultures with a higher sensitivity than the standard luminol-based assay.  More sensitive than traditional assays (use as few as 10e4 cells)  Enhancer is non-cytotoxic and non-denaturing  Up to 40-fold signal enhancement for cultured cells  Proprietary enhancer which increases signal at lea…
