Water soluble vitamin tablet analysis with HPLC

23 Mar 2022

This application note illustrates the separation of four hydrophilic B vitamins which were extracted from a commercially available tablet formulation. The data shows that these water-soluble B vitamins are well separated from each other and from any matrix peaks. The main advantage of this method over traditional reversed-phase analyses is that ion-pair agents are not needed to achieve acceptable results.

Cogent Diamond Hydride™ HPLC Columns

MicroSolv Technology Corp.

Cogent Diamond Hydride HPLC Columns are manufactured with TYPE-C™ silica (silica hydride) and a very small amount of carbon on the surface that adjusts the column hydrophobicity to an important level. This makes these columns have unique separation capabilities for scientists working in LCMS, metabolomics, bioanalytical, amino acids, organic acids, carbohydrates, and very polar small molecules.

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Water soluble vitamin tablet analysis with HPLC