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VistaPlex immunofluorescence assay panels

2 May 2024

VistaPlex Spatial Immune and Architecture Kits for spatial analysis on human formaldehyde-fixed, paraffin-embedded (FFPE) tissues enable the profiling of immune populations and tissue architecture using validated antibodies and optimized staining protocols. Canopy Biosciences details a study which compares the efficiency of different assay kits and highlights their ability to identify various cellular phenotypes across different tissue types and disease states. Further, Canopy Biosciences emphasizes the compatibility of VistaPlex kits with CellScape Whole-Slide Imaging Chambers, enhancing the reproducibility and utility of the assays in basic and translational research settings. Overall, VistaPlex kits offer a robust and cost-effective solution for spatial biology research, facilitating deep phenotyping of immune and cancer cells in diverse tissues.

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Fluorescence SpectroscopyFluorometers and spectrofluorometers (also called fluorescence spectrometers) are used to measure the intensity and wavelength of fluorescent light emitted from a sample after excitation by illumination. Spectrofluorometers utilize monochromators to select the desired wavelengths, whereas filter fluorometers employ a set of filters. Spectrofluorometers for measuring steady-state fluorescence and lifetime fluorescence (or time-resolved fluorescence) are available, as well as fluorescence microscopes and microplate readers. Find the best fluorescence spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Assay AssemblyAssay Assembly is technique used in drug discovery to develop assays to test the cytotoxicity, genotoxicity, or other activities of a compound on a cell. Assay assembly requires chip assembly, a delivery system and a detection and analysis method. Beneficial features of assay kits or automated systems include high-throughput, high speed and sensitivity and low signal to noise ratio.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.ImmunofluorescenceAssay DevelopmentThe process of proving an assay to be sensitive with respect to the target is known as assay development. The assay should be able to characterize novel compounds and measure the potency of these compounds against a validated biological target.Immunophenotyping