UV-traceable reagent for controlled biotinylation of antibodies

Discover a UV-traceable biotinylation reagent that enables controlled and non-destructive quantification of biotin incorporation into antibodies. Using Sulfo-ChromaLINK^® Biotin, researchers can measure the biotin molar substitution ratio (MSR) directly through a simple UV spectrophotometric scan, eliminating the need for indirect or destructive assays such as HABA or fluorescent binding assays. This white paper demonstrates predictable biotin labeling efficiency and shows that increasing MSR improves ELISA detection sensitivity, highlighting the importance of controlled antibody biotinylation for immunoassay optimization.