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Utilizing Mixed Mode μElution SPE for the LC-MS/MS Analysis of Steroid Hormones in Serum for Clinical Research

Utilizing Mixed Mode μElution SPE for the LC-MS/MS Analysis of Steroid Hormones in Serum for Clinical Research

30 Dec 2015

This poster evaluates an LC-MS/MS method used to measure serum testosterone and androstenedione enabling investigation of metabolic dysfunction for clinical research purposes. Testosterone, and its precursor, androstenedione, are androgenic steroid hormones that play a central role in the regulation of sexual characteristics.

Oasis® µElution Plate

Waters

The Oasis® uElution plate format combined with the Oasis chemistries allow elution volumes as low as 25uL, eliminating the time consuming evaporation step, producing very clean extracts with a 5 to 10 fold increase in concentration.The Oasis® family of sample extraction products is designed to simplify and improve your sample preparation by combining the right sorbent chemistry, device format and methodology. Achieve robust, selective, and sensitive solid-phase extraction [SPE] methods without worrying about low recoveries caused by breakthrough, sorbent drying, pH limitations, and undesirable silanol activity.Oasis® SPE sorbents—covered by eight U.S. patents—are unique in their purity, reproducibility, stability, and retention characteristics. Today, they are the most widely used polymeric SPE products in bioanalytical laboratories.

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