ResourceLife Sciences
Utility of Hybrid Multi-Mode Readers using Both Monochromator- and Filter-Based Detection Capabilities for the Measurement of ADP Accumulation
1 Sept 2015Multi-mode microplate readers are one of the more indispensable laboratory instruments used in life science research today. Advances in reader technology now allow for an incredible array of assays to be performed with readout based on the detection of a wide range of fluorophores, luminophores, and chromophores with spectral properties in the UV to IR. In this application note, three fluorescence-based assay technologies were used to test the capabilities of each detection system. The assays utilize Fluorescence Polarization, Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET), or Fluorescence Intensity for the measurement of ADP accumulation.
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Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Fluorescence Based AssayFluorescence based assays are widely used in life science research and high-throughput screening to measure a broad range of cellular activities.TR-FRETMuscleATP Monitoring