Using Inverted SPIM (iSPIM) to Study Neural Development in <i>C. elegans</i>

21 Nov 2014

This application note describes the use of a light sheet fluorescence microscopy technique, Inverted Selective Plane Illumination Microscopy (iSPIM), to image the neural development of the nematode C. elegans. iSPIM aenables very fast non-invasive imaging of living samples, facilitating the inspection of live neurodevelopmental events and paving the way for a 4D dynamic neurodevelopmental atlas.

iXon Ultra 897 EMCCD Camera

Oxford Instruments Andor

Facilitated by a fundamental redesign, the NEW iXon Ultra platform takes the popular back-illuminated 512 x 512 frame transfer sensor and overclocks readout to 17 MHz, pushing speed performance to an outstanding 56 fps (full frame), whilst maintaining quantitative stability throughout. The status of ‘Ultimate Sensitivity’ is also preserved in this model, offering thermoelectric cooling down to -100°C and industry-lowest clock induced charge noise. Additional unique features of the iXon Ultra include USB connectivity and direct raw data access for on the fly processing. EMCCD and conventional CCD readout modes provide heightened application flexibility, with a new ‘low and slow’ noise performance in CCD mode.  

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Using Inverted SPIM (iSPIM) to Study Neural Development in <i>C. elegans</i>