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True parallel protein purification with PhyTip® columns using the Freedom EVO® and MultiChannel Arm™ 96

Tecan
11 Jun 2015

This application note illustrates how high concentrations of fully functional protein can be obtained with small sample and elution volumes, producing high enrichment factors.

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MultiChannel Arm 96 (MCA96)

Tecan

True parallel processing for Freedom EVO® liquid handling platforms Tecan’s MultiChannel Arm module has a 96 channel pipetting head that brings higher productivity to almost any automated liquid handling process, from drug discovery and compound management to biotechnology or genomics applications. The module can be mounted onto the Freedom EVO 100, 150 or 200 platforms, increasing the efficiency and speed of your pipetting processes and delivering a greater level of flexibility to your platform. The MultiChannel Arm works in parallel with the other arms of Freedom EVO, for example, processing a set of plates while the robotic manipulator arm is bringing the next set onto the worktable. The MultiChannel Arm can also operate as an 8 or 12 channel head as desired, picking a column of 8 or a row of 12 tips to perform for example, in-plate serial dilution for IC50 assays. It works with various disposable and washable tips and can exchange them automatically during a run. MultiChannel Arm highlights are: true parallel processing compatible with all Freedom EVO options able to pipette with just 8 or 12 channels when necessary capable of switching tip types, even during a run integrates with a variety of modules Not all options are available in the US.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Antibody PurificationProtein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.

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