ResourceLife Sciences
Transiently Transfected Cell Lines for GABA Receptor Screening using the IonFlux System
16 Aug 2012Read this application note which describes an automated patch-clamp assay using transiently
transfected mammalian cells (HEK293T) with the IonFlux instrument (Fluxion Biosciences). The assay was validated by measuring the electrophysiological response of benzodiazepine-based compound HZ166. The use of transiently transfected cells allows for maximum flexibility for the assembly of different GABAA receptor types for screening purposes and the robustness of the developed assay makes it well suited for the high-throughput screening using the IonFlux HT system.
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ElectrophysiologyElectrophysiology is the study of biological voltage changes, from a single ion channel through to a whole organ scale. Voltages can be measured using patch clamping, automated workstations, electrophysiology rigs or cell based assays. Specific cell lines, manipulators and microinjectors are also available for electrophysiology research.TransfectionTransfection introduces nucleic acids (DNA or RNA) into cells, facilitating gene expression, genome editing, and functional studies. It�s widely used in genetic research, drug discovery, and therapeutic development. Find transfection reagents, kits, and systems in our product directory with reviews and pricing options.ReceptorsGABA