Tips, tricks and best practices for qPCR assay design

1 Jun 2021

In this qPCR assay design guide from Bio-Rad, discover top tips when designing primers and hydrolysis probes, expert advice for improving reproducibility and minimizing contamination, how to avoid the potential pitfalls of real-time PCR, and much more.

Reliance One-Step Multiplex RT-qPCR Supermix

Bio-Rad

Use the Reliance One-Step Multiplex Supermix for sensitive amplification of up to 5 targets in a single reaction. Expertly formulated with high-performance enzymes and advanced reaction buffer, this robust supermix delivers reproducible RT-qPCR performance in a multiplex fashion, including with challenging templates, difficult targets or in the presence of PCR inhibitors.

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Tips, tricks and best practices for qPCR assay design