ResourceLife Sciences
Tips & tricks to improve your immunohistochemistry and immunofluorescence staining
26 Feb 2026Optimize your immunohistochemistry (IHC) and immunofluorescence (IF) results with this comprehensive Staining Sample Preparation Guide from Vector Laboratories. Explore best practices for tissue storage, sectioning, deparaffinization, antigen retrieval, and hydrophobic barrier application to preserve morphology, maintain antigenicity, and reduce background staining. Whether working with FFPE or frozen samples, this guide provides practical tips, troubleshooting insights, and workflow recommendations to help you achieve consistent, high-quality staining results.
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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Cellular PathologyCellular Pathology deals with the microscopic analysis of tissue samples and cells. Sample preparation and processing includes fixation, staining, sectioning and slide mounting, using equipment such microtomes and cryostats. In choosing immunohistochemistry and immunocytochemistry kits, consider chromogens, staining method, antibodies, microscopes and imaging.ImmunohistochemistryImmunohistochemistry (IHC) is a technique used to detect specific proteins in tissue samples by using antibodies that bind to target antigens. IHC is widely applied in pathology, immunology, hematology and cancer diagnostics. Explore IHC tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.ImmunofluorescenceStaining