Immunofluorescence mounting guide

Mounting is a critical final step in immunofluorescence, directly influencing signal intensity, image clarity, and long‑term sample stability. This guide explains the principles behind fluorescence, the causes of photobleaching, and how mounting‑media selection affects tissue integrity and imaging quality across diverse applications.

Learn how antifade formulations, refractive‑index matching, and practical workflow tips can reduce photobleaching, improve image consistency, and support more reliable IF data for your research.