ResourceLife Sciences
Immunofluorescence mounting guide
20 Feb 2026Mounting is a critical final step in immunofluorescence, directly influencing signal intensity, image clarity, and long‑term sample stability. This guide explains the principles behind fluorescence, the causes of photobleaching, and how mounting‑media selection affects tissue integrity and imaging quality across diverse applications.
Learn how antifade formulations, refractive‑index matching, and practical workflow tips can reduce photobleaching, improve image consistency, and support more reliable IF data for your research.
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Fluorescence SpectroscopyFluorometers and spectrofluorometers (also called fluorescence spectrometers) are used to measure the intensity and wavelength of fluorescent light emitted from a sample after excitation by illumination. Spectrofluorometers utilize monochromators to select the desired wavelengths, whereas filter fluorometers employ a set of filters. Spectrofluorometers for measuring steady-state fluorescence and lifetime fluorescence (or time-resolved fluorescence) are available, as well as fluorescence microscopes and microplate readers. Find the best fluorescence spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell Staining