Thermo Scientific Phire Animal Tissue Direct PCR Kit: Genotyping Transgenic Mice

Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects. The Phire® Animal Tissue Direct PCR Kit employs Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA-binding domain that enhances the processivity of the polymerase. Phire Hot Start II DNA Polymerase also exhibits extremely high resistance to many PCR inhibitors found in animal tissues.The kit contains all the necessary components for amplification of DNA directly from animal tissues: optimized reagents, sampling tools, and DNARelease Additive, which can be used to improve the release of DNA from animal tissues. In addition, the kit includes detailed instructions and control primers that can be used with numerous animal species.Phire Animal Tissue Direct PCR Kit Features & Benefits: No need for time-consuming and expensive DNA purification steps Very little sample material required Two simple protocols for various applications Convenient sampling tools included in the kit Phire Hot Start II DNA Polymerase provides high yields of specific product with short extension time (20 s/kb). Minimized pipetting with master mix format and loading dyes pre-added Extremely short PCR protocol times Tested with wide variety of sample types Robust hot start DNA polymerases guarantee high yields of specific product Rich composition of the kit - controls, water and DNA ladder included Phire Animal Tissue Direct PCR Kit Includes: Phire Hot Start II DNA Polymerase 2X Phire Animal Tissue PCR Buffer (includes dNTPs and MgCl2) Universal control primer mix Dilution Buffer DNARelease Additive Gel loading dye Harris Uni-Core™ 0.50mm Harris Cutting Mat™