The Influence of pH on Extraction Efficiency with the Tecan AC Extraction Plate™
26 Apr 2015The AC Extraction Plate with TICE™ technology simplifies the sample preparation of biological samples. For efficient extraction from a biological sample matrix into the TICE coating, it is important that the molecule is in a neutral state. This technical note provides information on the pH-related factors that influence the distribution coefficient of a molecule between the TICE coating and the aqueous medium. It also explains how the optimal extraction pH can be determined experimentally, or be approximated by calculation.
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AC Extraction Plate™
TecanThe Tecan AC Extraction Plate* with TICE™ (Tecan Immobilized Coating Extraction) technology revolutionizes your sample preparation routine. The AC Extraction Plate is a 96-well, deep-well microplate, with each well having a proprietary TICE™ coating. TICE is a material with distinct absorptive properties, and this coating efficiently extracts specific small molecule analytes from aqueous solutions, releasing them when in contact with a suitable organic solvent. A simple pipette and shake sequence, with no filtration, centrifugation or solvent evaporation, is all that is required. The AC Extraction Plate is easily integrated into automated processes, making it a perfect match with Tecan’s Freedom EVO® liquid handling platform. AC Extraction Plate™ Features: Cost-effective – Simple pipette and shake workflow, without labor intensive centrifugation, filtration and solvent evaporation steps. Convenient and fast – Just unpack and start, no conditioning or other preparations needed. Highly reproducible – Pipette and shake routines are easily standardized and automated on a liquid handling platform, eliminating random errors and increasing consistency of results. * For research use only – not for clinical diagnostic applications