The ELF Preparative Electrophoresis System for Size-Based Proteome Fractionation
The ELF Preparative Electrophoresis System for Size-Based Proteome Fractionation
15 Oct 2015Although the resolution of LS-MS systems is improving every year, size fractionation of complex samples prior to MS analysis is still very useful for many proteomic studies. Despite the promise of automated 2D LC systems, SDS PAGE remains a popular, but laborious, pre-fractionation method for MS. This poster demonstrates an automated preparative electrophoresis system (the ELF system) to fractionate complex protein samples into 12 contiguous size fractions. The system uses a two-dimensional process: first, proteins are electrophoresed through an SDS agarose gel column to separate the proteins by size, and second, the separated proteins are electrophoresed sideways out of the agarose column into a linear array of membrane-bounded elution modules that are positioned alongside the separation gel column. The fractionated samples are recovered in SDS buffer, and can be processed for LC-MS using commercial detergent removal spin columns and standard trypsin digestion methods.
Related Products
Request Quote for All Products
SageELF Sample Fractionator for DNA and Protein
Sage ScienceThe SageELF is an automated, whole-sample fractionation system for NGS sample prep. SageELF, a whole-sample fractionation tool, is now shipping from Sage Science, developer of the Pippin automated DNA size selection system. The SageELF (short for Electrophoretic Lateral Fractionator) generates 12 contiguous fractions from a whole DNA sample, allowing scientists to generate libraries with multiple insert sizes from the same sample, which has applications in genome sequencing, structural variant detection, and identification of rare splice variants. This sample prep instrument will also be especially useful for preserving precious samples. Users simply load their sample, start the run, and pipette out the fractions later. Unlike in-gel digestion, in which some DNA fragments never leave the gel, the whole DNA sample is electrophoresed into the elution modules.Sage Science targets labor-intensive steps in the sample prep process where automation can offer improved precision and reproducibility. Precise size selection is critical for optimizing sequencing efficiency, improving genome assemblies, and reducing NGS project costs.Features: Make libraries with multiple insert sizes from the same sample Ideal for rare or precious samples Automated for precise, reproducible results Applications: Genome sequencing Structural variant detection Rare splice variant detection