ResourceLife Sciences
The effect of column hardware on the analysis of synthetic oligonucleotides by LC-MS
22 May 2023Therapeutic oligonucleotides represent a recent breakthrough in the pharmaceutical industry. Improved reliability to synthetic routes coupled with new drug delivery technologies has given a heightened level of interest in these clinically interesting targets. In this application note from Phenomenex, learn how by minimizing non-specific interactions, the use of BioTi™ hardware not only serves to improve chromatographic performance and reproducibility of oligonucleotide separations, but also maximizes analyte recovery and sensitivity enabling both consistent quantitation and characterization.
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OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.