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Targeted and Sensitive Detection of Food Allergens in Complex and Processed Foodstuffs Using UPLC-MS/MS

18 Apr 2017

This application note describes the targeted analysis of four food allergens in a variety of matrices using Waters ACQUITY UPLC System and Xevo TQ-S. The four allergens investigated in this method were milk (Bos Taurus), egg (Gallus gallus chicken), peanut (Arachis hypogaea) and soybean (Glycine Max [Glycine hispida]). The protocol was tested on processed and complex food matrices including chocolate, ice cream, tomato sauce, and cookies. Detection levels tested were benchmarked against the levels stated in the AOAC SMPR 2016.002 and VITAL (Voluntary Incidental Trace Allergen Labeling) reference doses.

ACQUITY QDa Mass Detector

Waters

The ACQUITY QDa® Mass Detector gives you certainty above all else. You can minimize the risk of unexpected coelutions or components and confirms trace components with the analytical confidence of mass detection. The ACQUITY QDa® Detector is a mass detector built around the needs of analytical scientists for chromatographic analysis. Robust, reliable and requiring no sample adjustments, it just integrates with your current LC, ACQUITY UPLC, ACQUITY UPC2 and purification systems. You'll enhance the analytical value and productivity of each analysis, and you won't need to run all the additional assays or time-consuming alternative techniques or even wait for results from specialist labs that cost your laboratory productivity. With the ACQUITY QDa® Detector, you now have information-rich mass spectral data to complement data from your current Waters® optical detectors, including the ACQUITY UPLC PDA, TUV, ELS, and FLR detectors, as well as the ACQUITY UPC² PDA Detector. This mass spectral information integrates seamlessly into the same workflow; routinely giving you more complete separation characterization. Features: Intuitive operation More information from every sample Effortless integration Increased efficiency Resolving complexity Applications: Food & Environment Life Sciences and Pharmaceuticals Chemical

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Targeted and Sensitive Detection of Food Allergens in Complex and Processed Foodstuffs Using UPLC-MS/MS