Successful Implementation of ChIP-seq Quality Control at Diagenode Using Automated ChIP Protocol on the SX-8G IP-Star® Compact
2 Dec 2014Chromatin immunoprecipitation (ChIP) is the most widely used method to study protein-DNA interactions. ChIP followed by HTS (ChIP-seq) enables the extension of the target directed analysis of ChIP results to a genome wide analysis. In view of the enormous potential and growing interest of this new development and to increase the quality standards of their antibodies, Diagenode has added this new technique to their QC procedure. This poster presents 19 ChIP-seq grade antibodies analyzed using the SX-8G IP-Star® Compact.
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SX-8G IP-Star® Compact Automated System
DiagenodeChromatin immunoprecipitation and DNA methylation assays followed by next generation sequencing is a powerful technique for generating genome-wide profiles of epigenetic modifications and transcription factor binding sites. The SX-8G IP-Star® Compact Automated System provides the flexibility needed to change multiple parameters in the protocols to achieve optimal experimental conditions. Samples generated on the system are compatible with popular downstream applications such as QPCR, microarrays and next generation sequencing platforms. In addition to automated ChIP and DNA methylation protocols, this system contains a wide variety of protocols including next generation sequencing library preparation and sample purification. Easy-to-use user interface for protocol optimization Magnetic-bead technology for great reproducibility Reduced "hand on time" to just only 15 minutes Easy to use bench-top liquid handling system Process up to 16 samples in parallel True "walk-away" automation Flexibility to adapt your experimental condition