Specific isotopic labeling of methyl groups has extended the molecular weight limits for NMR studies of protein structure and dynamics

Nuclear magnetic resonance (NMR) spectroscopy has emerged as the preeminent tool in solution studies of protein structure, dynamics, and intermolecular interactions. A key limitation, however, was that only relatively small proteins could be studied. In this application note, Cambridge Isotope Laboratories shares a brief summary of the ILV methyl group labeling schemes that are available and how these are utilized with current-day protein NMR methods.