Solving the Aggregation Problem of Human Embryonic Kidney 293 Cells Using the New Brunswick™ S41i CO<sub>2</sub> Incubator Shaker

25 Aug 2015

This application note shows a successful example of the adaptation of a human recombinant protein-expressing HEK293 cell line from serum-supplemented attachment culture to serum-free single cell suspension culture using the New Brunswick S41i CO2 incubator shaker. This adapted HEK293 cell line behaves like a typical aggregationless suspension cell line.

New Brunswick S41i Incubator Shaker

Eppendorf

For over a decade New Brunswick CO2 incubators have provided researchers with a reliable, easy-to-use system for culturing cells. Continuing this tradition is the New Brunswick S41i CO2 Incubator Shaker, designed specifically for non-adherent cell culture applications. The S41i combines precise temperature and CO2 control with the best laboratory shaker available. The result: high cell yields and viability. Like all New Brunswick CO2 incubators, the S41i is loaded with advanced features. A unique six-sided direct-heating profile provides gentle convection circulation of the chamber atmosphere for exceptionally uniform temperature control. But what makes the S41i really special is the built-in New Brunswick shaker. Combining our advanced CO2 incubator and shaker technologies creates a highly stable environment for reliable cell growth. When it comes to high cell yields and viability, as well as overall reliability, you won’t find a better solution. Features: Fanless design eliminates a common source of repeated contamination and expensive HEPA filters Sealed inner/outer doors and advanced PI control maintain temperature accuracy and uniformity while minimizing costly gas consumption High temperature disinfection (HTD) protects against bacterial contamination Unique InfraRed (IR) CO2 sensor offers specific measurement and accurate control of CO2 levels 25 mm access port for adding instrumentation or additional probes USB port for communication and external instrument logging Can be used on the floor, under counter or double stacked to save space; ideally sized for a cell culture lab Includes removable stainless steel humidity pans Optional BioCommand® SFI software for remote and historical data logging and report generation

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Solving the Aggregation Problem of Human Embryonic Kidney 293 Cells Using the New Brunswick™ S41i CO<sub>2</sub> Incubator Shaker