Simple Immunoprecipitation of Phosphorylated Proteins from Pervanadate Stimulated CHO Cells using Paramagnetic Beads

20 Jan 2011

This application note shows efficient concentration and enrichment of tyrosine phosphorylated proteins from mammalian cells. The Mag Sepharose technology allows capture of low abundant proteins from large amount of starting samples and elution in MS suitable volumes.

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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.
Simple Immunoprecipitation of Phosphorylated Proteins from Pervanadate Stimulated CHO Cells using Paramagnetic Beads