ResourceLife Sciences
Simple Enrichment of Phosphopeptides using TiO2 Magnetic Neads
24 Jul 2013Phosphorylation is a common, reversible post-translational modification involved in the regulation of many essential biological processes such as cell signaling, which is of prime importance for the study of various disease states such as cancer. Knowledge about phosphorylation may help to understand disease progression. Phosphoproteins and phosphopeptides are usually present at very low concentrations and ionize poorly, making their detection by mass spectrometry (MS) difficult. In this application note read how porous agarose TiO2 beads with paramagnetic properties can be used as a tool to enrich phosphopeptides.
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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer.
ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Cancer ResearchAlthough cancer is often referred to as a single condition, it actually consists of more than 100 different diseases. Microscopy, mass spectrometry, high throughput sequencing and flow cytometry are some of the most common techniques employed in cancer research labs.PhosphorylationProtein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.Magnetic Beads