Simple Conversion from ELISA to Luminex® Using the AMG™ Activation Kit from Anteo Technologies.

5 Jul 2015

Luminex® MagPlex® Microspheres may be coupled with antibodies specified for use in ELISA using Anteo Technologies’ AMG™ Activation Kit. This kit uses Mix&Go™ technology to strongly couple antibodies to multiplex microspheres such as Luminex® MagPlex® Microspheres. This application note gives the example of coupling two anti-cytokine antibodies to MagPlex Microspheres, followed by a multiplex immunoassay using the Luminex® system with the matched detection antibodies and standards.

MagPlex® Multiplexing Microspheres

Luminex

xMAP® Technology uses color-coded microspheres to detect up to 500 protien or oligonucleotide analytes from a single aliquot of sample in a single reaction well, enabling you to generate more data while saving sample, time, and reagents. Depending on your targets and the application, there are five different microsphere types to select from to produce the best experimental results. MagPlex® are magnetic carboxylated polystyrene microspheres that have been dyed into 500 spectrally distinct sets or “regions”, allowing them to be individually identified by an xMAP instrument. In the multiplex assay environment, the microspheres act as both the surface for the solution phase assay and the spectral identifier that the instrument detects. The open architecture of xMAP Technology enables users to build custom multiplex assays or select from a broad menu commercially available kits.  MagPlex are the most versatile xMAP microspheres and are: Compatible with both protein and nucleic acid applications Maximum xMAP microsphere regions available: 500 Superparamagnetic, eliminating the need for filter plates and offering superior washing efficiency Ideal for automated high throughput applications Available in two concentrations: 2.5 (10 regions only) & 12.5 (millions/mL) Available in multiple vial sizes: 1 mL, 4 mL, Custom Compatible with all xMAP instruments: FLEXMAP 3D® (500 regions), Luminex® 100/200™ (80 regions), MAGPIX® (50 regions) Additional xMAP microspheres: MagPlex-TAG™, MicroPlex®, MagPlex-Avidin®, SeroMAP™

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