Separation of Bovine Serum Albumin and Chicken Egg Lysozyme using PureSpeed Ion Exchange Tips

3 Nov 2014

This application note shows that ion exchange with the PureSpeed system is capable of yielding highly purified protein. Lysozyme is not efficiently retained by the strong anion exchange resin, while BSA is retained and purified. PureSpeed’s four different resin types, in conjunction with an E4 XLS 8- or 12-channel pipette make parallel purification of multiple proteins possible.

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Pipettes and DispensersPipettes and dispensers are essential lab equipment used to dispense measured volumes of liquid accurately. Explore a range of manual and electronic pipettes, including single and multichannel, serological and volumetric pipettes, as well as a range of pipette tips. Bottle-top dispensers are also available for simple and fast solvent dispensing. Find the best pipettes & dispensers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Separation of Bovine Serum Albumin and Chicken Egg Lysozyme using PureSpeed Ion Exchange Tips