This application note describes the acquisition of TIRF images using a broadband X-Cite® mercury light source without the cost of a laser and less photobleaching. This technique opens up advanced imaging to more researchers who want an affordable alternative that minimizes photobleaching associated with other technologies.
The X-Cite® 120PC Q system uses a 120-watt lamp to deliver rich spectral excitation with a uniformly illuminated field of view, the convenience of pre-aligned lamps, 2000 hour lamp lifetime, and adjustable iris - with the added benefit of being computer controllable. The straightforward RS-232 command set easily allows integration into automated systems as well as commercially available imaging software platforms. The X-Cite® 120PC Q system Includes: RS-232 communication; Compatible with Carl Zeiss, Leica, Nikon, and Olympus, Motic microscopes. Additional Specifications: Certifications: CE Marked, Certified to IEC, Canadian and US Standards
The X-Cite® 120Q system uses a 120-watt lamp to deliver rich spectral excitation with a uniformly illuminated field of view. The proprietary Intelli-Lamp® provides more than 2000 hours of life. The lamp is pre-aligned so changing it is a simple, one-step process. The optional adjustable iris allows the user to set the level of illumination. Ideally suited to routine fluorescence assays and use in multi-user facilities. The X-Cite® 120Q system Features: Lamp: 120W, Pre-aligned, Intelli-Lamp® technology, 2000 hour guaranteed life. Optional: Adjustable iris;Compatible with Carl Zeiss, Leica, Nikon, and Olympus, Motic microscopes. Additional Specifications: Certifications: CE Marked, Certified to IEC, Canadian and US Standards
The X-Cite® exacte employs a DC-powered mercury lamp coupled with patented Closed-Loop Feedback technology to provide ultra-stable illumination, for imaging protocols lasting from milliseconds to days. With the X-Cite® Optical Power Measurement System, light output can also be calibrated in absolute power units (watts), ensuring truly repeatable experiments. Features of the X-Cite® exacte: Lamp: 200W, Pre-aligned, Intelli-Lamp® technology, 2000 hour guaranteed life. Includes: Closed-Loop Feedback technology, Adjustable iris in 1% increments, Computer Control & GUI, Internal high speed shutter. Compatible with Carl Zeiss, Leica, Nikon, Olympus and Motic microscopes. Additional Specifications of the X-Cite® exacte: Certifications: CE Marked, Certified to IEC, Canadian and US Standards
The X-Cite® Optical Power Measurement System includes both the X-Cite® XR2100 Power Meter and the X-Cite® XP750 Objective Plane Power Sensor. Designed for measuring optical power in watts at the specimen level, the X-Cite® XP750 has a low, sleek profile that fits on the microscope stage and offers the versatility of measuring power output from an X-Cite® illuminator or any other epi-fluorescence light source.
The X-Cite® 200DC offers the ultimate combination of optical performance, short term stability and a built-in fast shutter. With the convenience and superior illumination uniformity found in all X-Cite® systems, the X-Cite® 200DC's intuitively easy-to-use design also includes light guide auto-detection to ensure optimized light coupling - every time. Novice and experienced microscopists will enjoy the flexibility provided by the smooth 0-100% intensity adjustment and expanded spectral range.
