ResourceLife Sciences
Running Kinase Assays in Kinetic Mode with the Transcreener® ADP2 Assay
25 Mar 2014This application note describes how to run the Transcreener® ADP2 assay in kinetic, or continuous mode. Using this mode of measurement reduces the number of experiments required to optimize conditions for a new enzyme; e.g., incubation time and temperature, enzyme concentration, etc. Running assays in real time also helps save on the cost of reagents and provides a check on assay linearity.
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Transcreener® ADP² Kinase Assays
BellBrook LabsThe Transcreener® ADP² Kinase Assay Kit universally detects ADP produced by any kinase or ATPase using a direct mix‑and‑read format, eliminating coupling steps. Available in FP, FI, and TR‑FRET formats with far‑red tracers to minimize interference, it's HTS-ready across 96‑, 384‑, and 1536‑well plates with robust Z' ≥ 0.7 performance.