RNA and Protein Extraction From Skin Tissue

28 Dec 2018

Isolating DNA and RNA from animal or human tissue is often a time-consuming process that yields low quality nucleic acid for analysis. This application note demonstrates full homogenization with various samples, including bone and tumors, using the FastDNA Spin and FastRNA Pro Green Kits, by MP Biomedicals.

FastDNA SPIN Kit™

MP Biomedicals

  Rapid Method of Isolating Pure Genomic DNA from a Wide Variety of Sources!   Isolate PCR-ready DNA from a variety of sample types No hazardous organic reagents are required The FastDNA™ SPIN Kit quickly and efficiently isolates genomic DNA from almost any sample (plant and animal tissues, cultured cells, bacteria, yeast, fungi, insects, etc). Up to 200 mg of tissue or cells are processed by the FastPrep-24™ 5G, FastPrep FP120™, FastPrep-96™ or SuperFastPrep-1™ with the Lysing Matrix A tubes. The kit includes 3 different chaotropic buffers for the homogenization of a wide variety of sample types and the released DNA is purified by a silica-based spin filter method. Purified DNA is ready for enzyme digestion, electrophoresis, PCR and any other desired application.

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