Reproducible DNA Amplification in PCR

Reproducible DNA Amplification in PCR

26 Mar 2016

Nowadays, PCR can be robust and easy—but you can still run into difficulties. Make sure to get the most out of your PCR with some simple tricks, with this informative infographic.

Mastercycler nexus X2 Thermal Cycler

Eppendorf

The Mastercycler nexus X2 gives you the ability to run two totally independent protocols at the same time. Smaller assays fit nicely on the 32-well-block — larger assays can exceed 48 samples and run on the 64-well-block. The larger block is available with a gradient function. As every member of the Mastercycler nexus family, the Mastercycler nexus X2 can be combined with all other family members in a network of up to 3 units. In combination with Eppendorf’s PCR Tubes, PCR Strips or divisible plates, the Mastercycler nexus X2 will give you consistent and publishable results — every day! Features: Large block for large assays — small block for small assays Intuitive graphic programming Universal block for strips, 0.1 mL, 0.2 mL and 0.5 mL PCR tubes Small footprint Optional gradient for PCR optimization E-mail notification Flexlid concept allows use of all types of consumables with automatic height adjustment of the lid Applications: Standard PCR Cycle sequencing Fast PCR Optimizing a PCR

(8)

Eppendorf Mastercycler Nexus PCR Cycler

Eppendorf

With its low noise level, the Mastercycler nexus can be used all day in the lab without disturbing any parallel work going on. Additionally, the low power consumption helps to save money and to be eco-friendly. Eppendorf Mastercycler nexus Family In the age of networking, the new Mastercycler nexus is your reliable companion when it comes to the daily routine of PCR. It is easy to use, does not need much space or energy and sends you an e-mail when it is done – what else do you need from a PCR cycler? Combine, Connect, Control ◾Combine up to 3 units for ultimate throughput! ◾Connect your Mastercycler nexus to your computer network and get a status e-mail to your desk! ◾Control all the relevant parameters of your PCR through the intuitive software Do you just need an affordable, flexible and reliable PCR instrument? The Mastercycler nexus is exactly that. It brings the reliability and ease of use of an Eppendorf cycler at an affordable price. Low energy consumption, easy expansion to 3 units and a small footprint make it not only fit to your budget, but also to your precious lab space. Due to the flexlid concept, the cycler can deal with all types of PCR consumables, from low volume all the way up to 0.5 mL PCR tubes. Of course, all common PCR plates fit – and if your throughput becomes even higher, you can just connect one or two Mastercycler nexus eco to your Mastercycler nexus. Gradient with SteadySlope Technology The gradient option of the Mastercycler nexus features the SteadySlope technology to ensure that the heating and cooling ramp rates are identical in both gradient and normal operation. Thus, you can expect identical temperature control characteristics in both optimization and routine experiments. Programming the gradient is easy and intuitive – so that novice users can get started quickly and safely. A true gradient with 12 different temperatures is an extremely powerful tool that can help you to optimize your PCR. Based on its recent analysis of the PCR instrumentation market, Frost & Sullivan recognizes Eppendorf with the 2012 European Green Excellence Award for its Mastercycler nexus.

(11)

Links

Tags

PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.qPCRQuantitative PCR (qPCR) is a molecular biology technique used to amplify and measure the amount of a specific DNA or RNA target. It plays a crucial role in gene expression analysis, pathogen detection, and quantifying DNA or RNA in various applications. Real-time PCR provides precise, quantitative results and is widely used in research, diagnostics, and clinical studies. Browse our peer-reviewed product directory to find the best qPCR solutions; compare products, check reviews, and get pricing directly from manufacturers.Sample HandlingProper and careful handling of samples is important in scientific studies to ensure the integrity of specimens. This will prevent deterioration and cross contamination, and improve the accuracy of sample tracking procedures.DNA AmplificationDNA Amplification is a technique used to amplify a single or multiple copies of DNA or mRNA by orders of magnitude. The most common method employed is PCR, but other options exist that eliminate the need for thermocycling.PCR PurificationReal-Time PCRReal-time PCR (RT-PCR) is a technique used to amplify and simultaneously quantify nucleic acids in real time. It is a key tool in gene expression analysis, pathogen detection, and diagnostics. RT-PCR allows for the detection of specific RNA or DNA sequences with high sensitivity and specificity. Browse our peer-reviewed product directory to find the best Real-Time PCR systems; compare products, check reviews, and get pricing directly from manufacturers.
Reproducible DNA Amplification in PCR