Removal of Beta-Glucuronidase Enzyme from Urine Post-Hydrolysis to Improve Assay Performance and Column Lifetime

Beta-glucuronidase is a 332 kDa enzyme that remains solubilized after incubation. Centrifuging samples post-hydrolysis is a common step to help remove the enzyme before analysis. This process works well in tubes, but the current centrifuges available for 96-well plates do not allow for sufficient speed to precipitate the enzyme. The increase in popularity of the 96-well plate format for high-throughput analysis has created a need for a simple technique to remove the enzyme that does not add significant cost to the assay. This application note demonstrates a simple post-hydrolysis step that removes the enzyme and significantly improves column lifetime.