ResourceLife Sciences
Real-time quantification of cell cycle phase in live-cell models
28 Apr 2021Cell cycle is a dynamic process and following this transition is critical in many therapeutic areas, such as oncology and immuno-oncology.
In this application note, discover how Sartorius’ Incucyte Cell Cycle Lentivirus reagents can be utilized with real-time image acquisition and analysis to provide a quantitative picture of this changing landscape.
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Incucyte® Live-Cell Analysis Systems
Sartorius GroupIncucyte ® , Empower Live-Cell Analysis Inside Your Incubator
Incucyte®️ Cell Cycle Green/Red Lentivirus Reagent (EF1α-Puro) – 0.2mL
Sartorius GroupOne vial of Lentivirus reagent (0.2 mL; >1x106 TU/mL) suitable for transducing cells with a non-perturbing, selectable cell cycle indicator. Stable cell lines can be created under puromycin selection.
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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Biopharmaceutical AdvancesBiopharmaceutical advances follow the development of pharmaceuticals derived from biotechnology, also known as biotechnology medicines. Biopharmaceuticals may be produced from cell lines, plants, or microbial cells. Important considerations of biopharmaceutical use include application, cost, production process and purification.Cell AnalysisThe analysis of cells allows researchers to understand the factors which contribute to cell health and function. These influencing processes can then be predicted and altered, leading to the development of medication and disease treatments.Cell GrowthCell growth refers to the process by which cells increase in size and number. It is essential for tissue development, wound healing, and cancer research. Understanding and controlling cell growth is a fundamental part of cell biology and regenerative medicine. Browse our peer-reviewed product directory to find the best tools for studying cell growth, compare products, check reviews, and get pricing directly from manufacturers.Live Cell ImagingLive cell imaging is the study of living cells using microscopes and high-content imaging systems. This technique provides in-depth insight into fast and complex biological processes, by allowing dynamic imaging of living cells instead of acquiring an individual image at a single point in time.Cell Cycle Analysis