Rapid qPCR Analysis from Highly Inhibited Tissue and Blood Sample Extractions with KAPA PROBE FORCE qPCR Kits and KAPA Express Extract

KAPA PROBE FORCE is a highly inhibitor resistant qPCR master mix that removes the need for DNA purification, enabling streamlined sample-to-Cq workflows. The master mix contains a third generation DNA polymerase evolved to overcome blood, tissue, and plant PCR inhibitors. Crude samples can now be analyzed with comparable accuracy, reproducibility, and sensitivity as purified DNA using KAPA PROBE FORCE.   Features:   Streamline sample-to-Cq workflows  in <1 hour. Eliminate the time and cost of sample purification by amplifying directly from crude samples. Analyze a wide range of sample types including  whole blood,  cells, mouse tails, FFPE, leaf, stem, seed, and soil. Generate accurate and reproducible results. Kits include a third-generation DNA polymerase, evolved for robust target amplification and detection. Enzyme maintains high reaction efficiency in the presence of PCR inhibitors for reliable data generation. Break through high levels of qPCR inhibitors. Achieve greater levels of sensitivity  for inhibited blood, tissue, and plant samples. Convert purified DNA assays to crude workflows without observable Cq delays. Multiplex crude samples efficiently. Accelerate genotyping analysis with single reaction allelic discrimination of crude DNA extracts. Maximize data collection from precious samples, increase throughput, and reduce costs.   Applications: GMO testing. Mouse transgenics. SNP genotyping. Food/water pathogen detection. Infectious disease research. Cancer research.

Rapid and efficient extraction of DNA from a broad range of source material. KAPA Express Extract is a novel thermostable protease and buffer system that allows for the extraction of PCR-ready DNA from various tissue types in as little as 15 minutes. The KAPA Express Extract system has been designed for optimal tissue lysis and DNA preservation. DNA extractions are conveniently performed in a single-tube, without the need for hazardous chemicals and multiple washing steps, thereby greatly reducing the risk of sample loss and contamination. Key features of the KAPA Express Extract system include: Rapid extraction protocol - PCR-ready DNA in 15 minutes Versatility - single kit optimized for a variety of sample types Single-tube reaction minimizes risk of contamination Couple with KAPA2G Robust HotStart ReadyMix for increased PCR success rates The combination of KAPA Express Extract and KAPA2G Robust HotStart ReadyMix provides the highest performance solution for rapid DNA extraction and consistent downstream amplification. KAPA2G Robust DNA Polymerase is a novel enzyme evolved for increased processivity and exhibits greatly improved tolerance to carryover PCR inhibitors from crude DNA extractions.