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Rapid Determination of Hesperidin in Orange Peel Using Accelerated Solvent Extraction and UHPLC
11 May 2016Hesperidin is an antioxidant that enhances the action of vitamin C to lower cholesterol levels. The Pharmacopoeia of the Peoples Republic of China (PPRC) 2010 recommends its extraction from fruits with a method that is both time- and solvent-consuming. The aim of this note is to develop a more efficient and cost-effective method to determine hesperidin in orange peel and other citrus fruits.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.FlavonoidsHesperidinPolyphenolic BioflavonoidNatural Herbal MedicineSolvent Extraction