RAFT 3D Cell Culture Viability and Proliferation Determination Using the CellTiter-Glo Luminescent Assay
30 Jan 2013It is a common requirement to assess the viability and proliferation of cells in culture and this is the same in 3D cell culture as it is in planar 2D culture. The CellTiter-Glo luminescent cell viability assay kit from Promega is a popular approach to this requirement in cell biology. In this technical note, it is shown that the standard protocol provided with the kit can be used without modification when some preliminary controls are used with the RAFT 3D cell culture system.
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RAFT (Real Architecture for 3D Tissue)
TAP BiosystemsRAFT is a new 3D cell culture system that enables scientists to create complex 3D tissue models in a simple, consistent and reproducible format. RAFT uses physiologically relevant collagen concentrations to create the most natural environment for cells. RAFT enables scientists to create 3D cell cultures in 3 simple steps in less than 1 hour. RAFT can be used to support assay development and secondary screening applications. It can also be used in a broad range of cell biology applications including: stem cell research, oncology research, toxicology and tissue engineering / scaffolds.