Quantifying Protein Concentration using UV Absorbance Measured by the Ocean HDX Spectrometer

10 Dec 2018

Proteins are a common sample in clinical, diagnostic and research labs. Studies involving protein extraction, purification or labeling, or working with proteins extracted from cells or labeled for study of the interactions between biomolecules, are common. Determination of protein concentration is a critical part of protein studies. In this application note, we use an Ocean HDX spectrometer to generate a standard curve for bovine serum albumin (BSA). High sensitivity in the UV, ultra-low stray light performance and upgraded optics make the Ocean HDX ideal for UV absorbance measurements.

Ocean HDX Raman Spectrometer - Compact, Affordable Spectrometer for 785 nm Raman Excitation

Ocean Optics

The Ocean HDX Raman spectrometer is a compact, high-performance spectrometer for 785 nm Raman excitation applications. This small-footprint instrument unlocks Raman signature data from 150 cm-1 to 3400 cm-1, is available with a 25 µm or 50 µm entrance slit, and can be combined with a laser, probe and sample holder to measure solids, powders and liquids. Applications range from authentication of spirits and analysis of cannabinoids, to identification of polymers and characterization

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Quantifying Protein Concentration using UV Absorbance Measured by the Ocean HDX Spectrometer