ResourceSpectroscopy

Quantification of Biomolecules: Absorbance or Fluorescence?

25 Jul 2017

Fluorescence and UV-­Vis absorbance are complementary measurement techniques for the quantification of biomolecules. Traditionally seen as separate, and sometimes competing methodologies, this article discusses the advantages of each, as well as new systems that integrate multiple modes into single, compact bench­top instruments.

DS-11 Series Spectrophotometer / Fluorometer

DeNovix

DeNovix takes spectrophotometry to a whole new level of performance with the DS-11 Series. This easy-to-use, compact spectrophotometer delivers full spectrum UV-Vis analysis and fluorescence capability, making it ideal for rapid nucleic acid and protein quality control.

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QFX Fluorometer

DeNovix

The DeNovix QFX Fluorometer offers the widest dynamic range, greatest sensitivity, and ground-breaking ease of use. The QFX includes four excitation sources and requires as little as 1 µL of sample. The system is compatible with DeNovix dsDNA and RNA Fluorescence Quantification assays, commonly used Thermo Fisher Qubit™ assays, or user-configured custom assays.

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DS-C Cuvette Spectrophotometer

DeNovix

The DeNovix DS-C Spectrophotometer offers outstanding dynamic range, sensitivity, flexibility, and ease of use. The instrument is ready right out of the box—no PC setup or software installation is required. From GMP compliance facilities to teaching labs, the versatile DS-C makes absorbance measurements simple with application-specific EasyApps™ software.

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Quantification of Biomolecules: Absorbance or Fluorescence?