Push-Button Simplicity: Automatic Fermentation with the BioFlo® 120 Auto Culture Mode

3 Feb 2017

The Auto Culture modes, standard on the BioFlo 120 bioprocess control station, offer push-button automatic process control for some of the most common microbial (E. coli) and mammalian (Chinese Hamster Ovary, CHO) cultures. In this application note, an E. coli batch fermentation was conducted using an Auto Culture mode to demonstrate this novel feature of the BioFlo120 control station.

Eppendorf BioFlo® 120

Eppendorf

The Eppendorf BioFlo 120 offers simplicity and ease of use, without sacrificing capability. In academic, governmental or industrial research settings; for work with bacteria, yeast, fungi, mammalian, insect or plant cells: The BioFlo 120 is an attractive solution to meet bioprocessing needs. The BioFlo 120 is a bench-scale bioreactor/fermentor system that perfectly suits research and development. Its flexible design meets the wide-ranging needs of scientists in both microbial and cell culture applications. 24 interchangeable autoclavable vessels (water-jacketed or with heat blanket) are available, along with BioBLU® single-use bioreactors ranging from 250 mL to 40 L working volume. Digital Mettler Toledo® ISM sensor technology is fully integrated allowing the user to switch between pH, redox, dissolved oxygen (polarographic and/or optical), and carbon dioxide monitoring without the need for additional equipment. A high-precision thermal mass flow controller with expanded range provides the capability for low flow mammalian cell culture through high-demand microbial processes on a single control station.   Features: Scale-up from 250 mL to 40 L on a wide variety of autoclavable and single-use vessels New Auto culture modes for microbial and cell culture process control at the touch of a button. Universal connections for analog sensors or digital Mettler Toledo ISM sensors for increased flexibility Applications: Cultivation of mammalian, stem, insect, and plant cells Bacterial, yeast, and fungal fermentation Batch, fed-batch, continuous or perfusion

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