Protocol for Fed Batch, Serum Free Cultivation of CHO XM 111 Suspension Cells in the BIOSTAT CultiBag RM 20
8 May 2011In this application note, Sartorius describe a protocol for the propagation of the model protein secreted alkaline phosphatise expressing CHO XM 111 suspension cells (obtained from Prof. Dr. Martin Fussenegger, Swiss Federal Institute of Technology, Zurich) in selective, chemically defined, protein and peptide-free ChoMaster media (HP-1 and HP-5) using the disposable bioreactor BIOSTAT CultiBag RM 20 basic.
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BIOSTAT® CultiBag RM single use bioreactor
Sartorius GroupBIOSTAT® CultiBag RM single-use bioreactor for the cultivation of all cell types, including mammalian cells, plant cells, insect cells, stem cells and microbial cells. Application-driven packages are available for culturing from 100 ml to 300 l. BIOSTAT® CultiBag RM is a single-use bioreactor utilising rocking motion agitation with low shear. The feedback control loops using single use sensors for pH and DO, bring new levels of confidence to process development.