Performing real-time activation and subsequent mitochondrial stress test of macrophages on the CYRIS analysis platform

21 Jul 2022

In this application note, INCYTON describes the preparation, performance, and results of a real-time activation and subsequent mitochondrial stress test of adherent cells of the mouse macrophage cell lines J774A.1 in the CYRIS® analysis platform.

CYRIS flox

INCYTON GmbH

CYRIS monitors and analyzes several key cellular parameters in real-time and label-free. Cell viability, metabolism, and morphology can be screened in a fully automated and atmosphere-controlled environment. The incubator integrated solution measures oxygen, pH, and cellular impedance in a multiwell plate and makes through a digital inverse microscope phase-contrast like live-cell images.

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Performing real-time activation and subsequent mitochondrial stress test of macrophages on the CYRIS analysis platform