Peptide Separation on Micro Pillar Array Columns (μPAC™). Part 1: Cytochrome C Tryptic Digest

24 Jan 2018

Reversed phase cytochrome C tryptic digest separations, are routinely employed for the analysis/quality control of high performance liquid chromatography column performance towards proteomics applications. The focus of this study is to illustrate the performance and versatility of a 200 cm µPAC™ column for peptide separations.

200 cm µPAC™ column

PharmaFluidics

As an alternative to conventional columns, PharmaFluidics offers micromachined nano-LC columns or micro Pillar Array Columns (µPAC™). These µPAC™ columns display exceptional separation properties with unprecedented peak capacity and narrow peak shapes at low operating back-pressure. µPAC™ column, 200 cm length, with pillar array backbone at interpillar distance of 2.5µm, C18.

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50 cm µPAC™ column

PharmaFluidics

With an internal volume of 3µL, this 50 cm long RP-C18 µPAC™ column is perfectly suited to perform high throughput analyses with shorter gradi­ent solvent times (30, 60 and 90-minute gradients) and it can be used over a wide range of flow rates, between 100 and 2000 nL/min. µPAC™ column, 50 cm length, with pillar array backbone at interpillar distance of 2.5µm, C18.

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Peptide Separation on Micro Pillar Array Columns (μPAC™). Part 1: Cytochrome C Tryptic Digest