ResourceLife Sciences
Optimized urine liquid biopsy workflow: From sample collection to cfDNA stabilization and purification, ready for digital PCR analysis
4 Feb 2025Cell-free DNA (cfDNA) in urine is an avenue for liquid biopsy research. In addition to being a standalone source for biomarkers, urine cfDNA has been shown to provide complementary information to blood cfDNA. Explore a preanalytical workflow, from urine collection and cfDNA stabilization to the isolation of high-quality unmodified cfDNA, allowing reliable urine cfDNA analysis with the QIAcuity Digital PCR system from QIAGEN.
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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.UrineThe analysis of urine is important in medicinal, anti-doping, forensic and life sciences applications. The ability to evaluate what someone has ingested through their urine enables fast detection and diagnosis of certain illnesses. Liquid BiopsiesLiquid biopsies are non-invasive tests used to detect cancer and other diseases by analyzing blood or other bodily fluids. This technique is gaining popularity for early detection, monitoring treatment response, and detecting minimal residual disease. Explore liquid biopsy solutions in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.cfDNAdigital pcr