Online SPE/LC-MS Method for the Rapid Analysis of Thyroid Hormones in Plasma
14 Jul 2014Thyroid hormones play critical roles in the regulation of biological processes such as growth and brain development. For the clinical laboratory, measurements of total T4 and total T3, along with estimates of free T4 and free T3 are important for the diagnosis and monitoring of thyroid diseases. An online SPE/LC-MS method was developed for the determination of the thyroid hormones T4, T3, and rT3 from plasma using Ascentis® Express RP-Amide cartridge to trap the analytes, and an Ascentis® Express Phenyl-Hexyl column to resolve them.
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Ascentis® Express RP-Amide HPLC Columns
Sigma-Aldrich SupelcoAscentis Express RP-Amide HPLC columns are the most recent product additions to the Supelco HPLC product line. Combining an embedded polar group (EPG) stationary phase with the Fused-Core particles, Ascentis Express RP-Amide provides a host of useful benefits to the HPLC chromatographer. Ascentis Express RP-Amide is a blend of modern phase technology and innovative particle technology. The Fused-Core particle provides benefits in terms of speed, resolution, sensitivity, and ruggedness. The one-step RP-Amide bonding chemistry provides benefits in terms of selectivity, aqueous stability, and improved peak shape for bases. Read about how this product was used to analyze Riboflavin and impurities here. Benefits: Alternative reversed-phase selectivity to C18 Improved peak shape for bases 100% aqueous compatible reversed-phase column Column Details: Chemistry: RP-Amide Particle Size: 2.7, 5.0 μm Pore Size: 90 Å Length: 20, 30, 50, 75, 100, 150, 250 mm ID: 2.1, 3.0, 4.6 mm