On-Cell Western Plate-Based Assay for Targeted NIR-Labeled Optical Imaging Agent Development

15 Mar 2018

This application note describes a protocol for receptor-based binding and competition assays using A431 (epithelial carcinoma) cells. Experimental considerations are also discussed including scan settings for the Odyssey System and microplate selection.

Odyssey DLx Infrared Imaging System

LICORbio

The Odyssey DLx Imaging System delivers accurate, reproducible, and quantitative data with near-infrared fluorescence. It supports Western blots, In-Cell Western™ Assays and other plate-based assays, cell-based assays, EMSAs, DNA and protein gels, protein arrays, and tissue section imaging. Discover more with the groundbreaking Odyssey technology cited in over 120,000 peer-reviewed publications.

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Odyssey M Imaging System

LICORbio

The Odyssey M is the most versatile and sophisticated biomolecular imager on the market. With 19 available channels, industry-leading 5 µm resolution, >6 logs of dynamic range, and an onboard luminescence module, the Odyssey M gives researchers a multimodal imaging solution with unparalleled capabilities and performance. Discover more with the groundbreaking Odyssey technology cited in over 40,000 peer-reviewed publications.

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