Novel Instrument for Electrophysiological Guidance of Nanoliter Injections in the Brain
12 Nov 2014In this poster, the Nanoject II was successfully modified to record extracellular neural activity. The Nanoject II uses a direct drive, a stainless steel piston inside a fluid filled pipette, and can inject as little as 2.3nl. The experiments tested this new instrument for electrophysiological localization of the gustatory responsive area of the parabrachial nucleus. The effects of small excitotoxic lesions suggest a functional topography within the gustatory parabrachial nucleus.
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Nanoject II Auto-Nanoliter Injector
Drummond Scientific Co.Features of the Drummond Scientific Nanoject II Auto-Nanoliter Injector include: • Automated Microprocessor controlled Microinjection Pipet delivering precise nanoliter volumes accurately • Remote control and nonrotating plunger eliminates vibration allowing precise injection minimizing possible cell rupture from microinjector• Selectable Injection Volumes: from 2.3 nanoliters to 69.0 nanoliters in 16 increments • Supplied with sufficient N-51-A glass capillaries for preparing 400 injection tips, standard softening point 780°C • Can be mounted on most micromanipulators (Note: May require Universal Adapter 3-000-024-A. The Universal Adapter is now included with the Nanoject) • Separate Foot Switch available to allow hands-free operation • Selectable Operating Speeds: Fill Speed Fast: 46 nL per second, Slow: 23 nL per second Injection Speed Fast46 nL per second, Slow: 23 nL per second Empty Speed Fast: 230 nL per second, Slow: 92 nL per second