ResourceLife Sciences
Northern Blot Analysis Using Biotin PCR Labelled Probes
20 Jun 2011This application note from LI-COR discusses methods for Northern blotting analysis, biotin probe labelling using PCR amplification, Northern blot hybridisation, biotin detection for Northern blots, and a troubleshooting guide.
Related products
Request Quote for All Products
Odyssey Fc Imaging System
LICORbioThe Odyssey Fc System is the only imaging system that offers superior chemiluminescent detection and the benefits of quantitative infrared Western blot detection as well as the ability to document DNA gels using the 600nm channel.
Odyssey CLx Infrared Imaging System
LICORbioThe Odyssey® CLx Infrared Imaging System gives you accurate, reproducible quantitative Western blotting results with near-infrared fluorescence.
Links
Tags
Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gel Doc / Image AnalysisGel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Explore a range of a gel imaging systems, densitometers, scanners, transilluminators or UV lamp + CCD cameras for your image analysis solutions. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis. See gel doc / Image analysis software for quantitative 1D and 2D analysis of your samples. Find the best gel doc / image analysis products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :