Normalization Accuracy for Western Blotting

19 Mar 2015

The normalization of data to an internal control is critical for meaningful quantitative analysis. Small changes in protein expression can have a huge impact on data interpretation. This white paper discusses normalization controls and how they can affect the accuracy of your Western blots. The use of housekeeping genes and choice of detection methods are also evaluated.

IRDye 800CW Infrared Dyes

LICORbio

Imaging probes conjugated to IRDye 800CW and IRDye 700DX have propelled more than 20 Phase I and Phase II clinical trials worldwide.

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IRDye 680LT Infrared Dyes

LICORbio

IRDye 680LT dye is highly soluble in water and is significantly brighter and more photostable than many other 700 nm near-infrared dyes.

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