NIR RPPA Detection Together with Dynamic Range Extension (XDR) Function in the Innoscan 710-IR Scanner Makes RPPA Detection Cheaper By 90 Percent

12 Nov 2014

Reverse Phase Protein Arrays (RPPA) also named cell lysate arrays have been shown to be useful tools for biomarker evaluation in large patient cohort. One of the challenges of this technology is the dynamic range, since biomarker expression levels can vary from fento-molar (fM) to micro-molar (μM) concentration in the same cohort. This application note demonstrates how, using the dynamic range extension (XDR) function in the InnoScan 710-IR scanner, RPPA costs are dramatically diminished when switching from colorimetric to fluorescence detection.

Advanced Infrared 2-colour Microarray Scanner: InnoScan 710IR

Innopsys

Unmatched performance for protein assays.Specifically designed to enhance detection in nitrocellulose protein array applications, the InnoScan 710IR near infrared (NIR) fluorescence scanner from Innopsys has on-board 670 nm and 785 nm excitation lasers to optimize performance by reducing the inherent background from the nitrocellulose. No scanner comes close for optical quality and speed of operation. Full compatibility with traceability standards is guaranteed, with a validation slide and associated Mapix software provided.Eliminating one of the most common concerns, the InnoScan 710IR is insensitive to substrate distortion. The real-time autofocus system makes the entire reading area completely uniform.Sensitivity and signal-to-noise ratio are two of the outstanding characteristics upon which the reputations of InnoScan scanners have been built. Outstanding photometric properties, combined with unique data manipulation software, ensure the most advanced analyses possible on the market for this class of scanner.Features: 670 nm and 785 nm excitation lasers for enhanced IR performance Simple to understand and operate – concentrate on the interpretation High precision - maximum resolution of 3 µm/pixel Under 4 minutes reading time combined with 10µm resolution Unbeatable scanning speed (10 to 35 lines/sec) Barcode identification for reliable traceability Portable, compact design; can be shared by labs Attractive price

(0)

Links

Tags

NIR RPPA Detection Together with Dynamic Range Extension (XDR) Function in the Innoscan 710-IR Scanner Makes RPPA Detection Cheaper By 90 Percent