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Multiplexing DyLight Infra red 680 and 800 dyes

Syngene
15 Nov 2018

Life sciences research has recently seen a trend towards the application of fluorescence-based detection of proteins on Western blots. Using fluorescence based fluorophores increases sample throughput and can make multiplexing several fluorophores less time consuming. Near-infrared has been shown to significantly reduce background autofluorescene and this has therefore generated a trend towards NIR fluorophores such as, IRDye® 680LT and IRDye® 800CW from LI-COR® and DyLight® dyes from ThermoFisher which provide enhanced detection sensitivity and quantification of proteins. The DyLight dyes offer intense signal whilst maintaining excellent photo-stability and water solubility across a broad range of pH values. Another advantage of using DyLight dyes is that they are competitively priced and permit the use of lower conjugate concentrations to be used.

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Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.FluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.ImagingImaging techniques are essential for obtaining visual representations of samples to understand structures, processes, and function in biological, chemical, and physical research. These tools range from traditional light microscopy to advanced imaging modalities like MRI and electron microscopy, providing researchers with valuable data for diagnostics, drug discovery, and material analysis. Explore imaging solutions in our peer-reviewed product directory to compare products, check reviews, and get pricing directly from manufacturers.MultiplexingMultiplexing refers to the ability to measure multiple targets or analytes simultaneously in a single experiment. This technique is valuable for high-throughput screening, diagnostics, and complex assays, as it increases efficiency and data quality. Browse our peer-reviewed product directory to find the best multiplexing products, compare tools, check reviews, and get pricing directly from manufacturers.

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