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Multiparametric Flow Cytometric Evaluation of Cytotoxic T Lymphocyte Populations

Beckman Coulter Diagnostics

Multiparametric Flow Cytometric Evaluation of Cytotoxic T Lymphocyte Populations

18 Mar 2016

Cytotoxic T lymphocytes (CTL) are the principal cells of the acquired immune response for defense against viral infections and tumors. The accurate analysis of such a functional effector population is critical to the elucidation of correlates of protection for diseases involving cellular immunity. Once standardized and validated, these assays can be routinely used as surrogate markers of efficacy in preventative and therapeutic strategies involving immune response. Multiparametric flow cytometry has enabled the accurate identification and evaluation of targeted lymphocyte subpopulations. In this poster, the functional capacity of effector T cells was evaluated using 5 color, 7 parameter flow cytometry by interrogating a combination of phenotypic and functional surface and intracellular markers.

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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :ImmunoassayImmunoassays are laboratory tests that utilize antibody-antigen interactions to detect specific substances. They are widely used in diagnostics for detecting diseases, drug testing, and environmental monitoring. These assays offer rapid, sensitive, and precise detection of biomarkers, making them invaluable in clinical and research labs. Browse our peer-reviewed product directory to compare immunoassay systems, read user reviews, and get pricing directly from manufacturers.CytotoxicityCytotoxicity assays measure the toxic effects of substances on cells, often used in drug testing and environmental studies. These tests are crucial in determining the safety of chemicals and pharmaceutical compounds. Explore cytotoxicity testing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Flow ChemistryFlow chemistry describes a chemical reaction being carried out continuously rather than in a batch process. The reagents are pumped through a pipe and forced together at a mixing junction, where they are allowed to combine until the reaction is stopped.LymphocytesImmunophenotypingTherapeuticsTherapeutics are treatments designed to alleviate or cure diseases. These include pharmaceuticals, biologics, and gene therapies, which work by targeting specific disease mechanisms. Advances in personalized medicine and biologics offer new hope for patients with conditions that were previously difficult to treat. Browse our peer-reviewed product directory to find therapeutic solutions for various diseases; compare products, check user reviews, and get pricing directly from manufacturers.Biomarker DiscoveryClinical biomarkers refer to substances related to known medical conditions that can be accurately measured <i>in vitro</i>. Biomarkers can be used to diagnose presence of a disease and indicate disease severity. The discovery of new biomarkers is incredibly valuable in the field of diagnostics.

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Multiparametric Flow Cytometric Evaluation of Cytotoxic T Lymphocyte Populations