This white note compendium by Waters explores multi-dimensional chromatography, focusing on the differences between loop and trap injection. When new technology is introduced, new options and combinations of analysis become available, often enhancing complex analyses. This white paper explains the differences between the two methods and how resolution can be optimized for analyses.
The rugged, robust and reliable Xevo TQD – a solid performer for clinical analyses
UPLC Technology Extends To Two Dimensions For chromatographers who require additional capabilities to increase their speed of analysis, gain sensitivity and selectivity, and perform orthogonal separations, ACQUITY UPLC® Systems can meet these needs by controlling multiple valves and pumps for 2D separations. The resolution, sensitivity, and throughput benefits of UPLC® Technology are even more important in 2D applications. Waters’ 2D Technology solutions for the ACQUITY UPLC, ACQUITY UPLC H-Class, and ACQUITY UPLC H-Class Bio systems are purpose-built, from plumbing to software to valve control, to provide reproducible and consistent results for specific applications. Features: • Ready-to-use configurations to get you to successful 2D UPLC experiments faster, with less troubleshooting and more confidence • ACQUITY UPLC systems and proven columns enable best-in-class selectivity and sensitivity
ACQUITY UPLC® HSS (High Strength Silica) T3 Columns contain the first and only 100% silica particle designed, tested and intended for use in applications up to 15000 psi/1000 bar. The ACQUITY UPLC® HSS particle is not an HPLC particle. High pore volume HPLC particles do not possess the mechanical stability necessary to withstand the high column packing and operating pressures of UPLC® separations. The first ligand chemistry in this UPLC®-certified column family utilizes T3 bonding in order to retain and separate polar organic compounds. ACQUITY UPLC® HSS T3 columns possess the superior polar-compound retention, aqueous mobile phase compatibility and ultra-low MS bleed of Atlantis® T3 columns. Easier Method Transfer Since Atlantis® T3 HPLC and ACQUITY UPLC® HSS T3 columns utilize T3 bonding, LC methods can be transferred from HPLC to UPLC® separations. Because Atlantis® and HSS particles are different substrates, some method modification and/or optimization may be required. A compelling benefit of UPLC® technology is the ease and speed at which this method optimization can occur since UPLC® separations offer greater resolution in less time. Moving from XBridge™ HPLC to ACQUITY UPLC® BEH columns is nearly seamless, as the hybrid particle substrates differ only in particle size. However, because of T3 bonding it should be straightforward to make simple adjustments to successfully transfer a separation from an Atlantis® T3 HPLC column to an ACQUITY UPLC® HSS T3 column.
